Technical Specifications

Mammalian Cell CountingYeast Cell Counting

Applications The NucleoCounter counts cell nuclei, stained with the DNA-specificfluorescent dye propidium iodide (PI).Recommended for use inpharmaceutical/biotechnology research & production facilities. Loading Volume 100 µL is recommended to ensure a representative sampleApproximately 50 µL is loaded into the cassette Analysis Volume Approximately 2 µL of the sample loaded into the cassette isanalyzed in the NucleoCassette chamber Analysis Time After pressing “Run”, the result will be displayed in about 30 seconds Measurement Range 5 x 10
3 to 4 x 10
6 cells/mL lysate5 x 10
3 to 2 x 10
6 cells/mL lysate Optimal Range 1 x 10
5 to 2 x 10
6 cells/mL lysate1 x 10
4 to 2 x 10
6 cells/mL lysate Operation Menu-controlled by means of keyboard and LCD display Dimensions 15”L x 8.7”D x 10.2”H Weight 6.6 lbs Power Requirements Input:120 V, 50/60 Hz120 V, 50/60 Hz Power Consumption Peak:25 Watt25 WattReady mode:10 Watt2.5 WattStandby:2.5 mWatt2.5 mWatt Operation Conditions For Indoor use Temperature 15°- 35°C (59°- 95°F) Relative Humidity Maximum 80% RH at < 31°C, decreasing linearly to 65% at 35°C The NucleoCassette™Reagent Approximately.2.8 µg propidium iodide storage Best stored in a sealed bag at maximum 30°C (86°F). Stability Stable for 12 months NucleoView™Software Requires Windows 2000 operating system, Windows-compatible computer & 1.1 USB port Ordering InformationNucleoCounter Catalog No.M1293-0000Catalog No.M1293-1000 NucleoCassettes Catalog No.M1293-0100.Sold in quantities of 100 cassettes (10/bag) NucleoView software Catalog No.P0540-5440 starter kit, Catalog No.M1293-0020 One bottle each lysis and stabilization buffer, Mammalian Cell each lysis and stabilization buffer, 2 dispensing pumps, reagent holder, Counting Only 100 NucleoCassettes and NucleoView software reagent YC-100 Catalog No.P0820-5185 - for yeast cell counting only
NucleoCounter®, NucleoCassette™and NucleoView™are trademarks of ChemoMetec A/S, Denmark.The information contained here-in is to the best of our knowledge accurate and complete.However cell species and cell environments may vary in property.Thereforesystematic and/or random deviation between estimates obtained by the NucleoCounter and other cell counting methods may occur.As such, nothing contained or stated herein, including results obtained from use of the NucleoCounter or NucleoCassette, shall be construedto imply any warranty or guarantee.New Brunswick Scientific (NBS) and affiliated companies shall not be held liable for damages, andcustomers shall indemnify NBS and affiliated companies against liability flowing from use of potentially inaccurate data generated by theNucleoCounter.It is recommendedthat all results obtained with the NucleoCounter be validated against appropriate reference methodsand/or traditional laboratory methods at regular intervals.

New Brunswick Scientific

P.O. Box 4005 • 44 Talmadge Rd. • Edison, NJ 08818-4005 USA800-631-5417 • 732-287-1200 Fax: 732-287-4222E-Mail: bioinfo@nbsc.com • www.nbsc.com

B3003a10K.P205