differential scanning calorimeter (DSC) - MicroCal LCC - #1

Differential Scanning Calorimetry (DSC) is unsurpassed as a method to determine the stability of biological systems. DSC directly measures heat changes that occur in biomolecules during a con­trolled increase or decrease in temperature, making it possible to study materials in their native state.

The MicroCal VP-DSC is the most sensitive, easy to use differential scanning calorimeter available to study samples in solution. It can be used to meas­ure the intramolecular stability of a broad spectrum of biomolecules, including proteins, nucleic acids, lipids and detergent micellar systems. The VP-DSC provides fast, accurate transition midpoint (Tm) determination. In addition, a thermodynamic profile can be generated, providing insight into the factors that affect conformation and stability.

Other applications include: determination of protein stability and folding, antibody domain structure determination, characterization of membranes and lipids, and the measurement of ultra-tight molecular interactions.

MicroCal instruments are found at major pharmaceutical, biotech, academic and overnment institutions worldwide.

Why DSC?

■ Save time and money in stability testing.

■ Study molecules in their native state without labeling. Can be used with solutions that interfere with optical methods including turbid or colored solutions or particulate suspensions.

■ Provides insights into mechanisms of unfolding and refolding.

■ Determine ultra-tight binding constants not able to be measured by other techniques (up to 10²⁰M^-1).

■ Easy to use: Unattended operation after sample loading.

■ Complete system: No additional accessories to purchase. No reagents or consumables are required.

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