Modulated LED Light Source For Fluorescence Lifetime Imaging - Lambert Instruments - #1

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Turfweg 4 9313 TH Leutingewolde The Netherlands Tel: +31-(0)50-501 84 61 Fax: +31-(0)50-501 00 34 E-mail: info@lambert-instruments.com Website: www.lambert-instruments.com Lambert Instruments Modulated LED Light Source For Fluorescence Lifetime Imaging The modulated Light Emitting Diode (LED) is used as excitation light source in systems for Fluorescence Lifetime Imaging Microscopy (FLIM) that operate in the frequency domain. Modulated LEDs of different wavelength bands are available. The output light intensity can be varied over a wide range and the maximum excitation power available at the sample is comparable with that of other light sources. The system consists of an RF-shielded LED assembly that is fitted in the standard mercury lamp housing as provided by the microscope suppliers Nikon, Olympus, Leica and Zeiss. The LED assembly is connected via a shielded cable to the Power Supply/Control Unit (PS/CU) that provides the DC and AC power to the LED. To build a complete set-up for lifetime imaging, a compatible modulation signal generator (control of modulation frequency and amplitude), modulated intensified CCD camera and dedicated image processing software are available from Lambert Instruments. I N F O R M A T I O N NEW