Lambert Instruments Modulated Intensifier II 18MD for Fluorescence Lifetime Imaging - Lambert Instruments - #1

Turfweg 4 9313 TH Leutingewolde The Netherlands Tel: +31-(0)50-501 84 61 Fax: +31-(0)50-501 00 34 E-mail: info@lambert-instruments.com Website: www.lambert-instruments.com

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PERFECTION IN IMAGE DETECTION

Lambert Instruments Modulated Intensifier II18MD For Fluorescence Lifetime Imaging

The Lambert Intensifier II18MD is a modulated image intensifier that is used for Fluorescence Lifetime Imaging Microscopy (FLIM) in the frequency domain. The product is designed for users who wish to apply the highest quality detector as part of their set-up for lifetime imaging.

The II18MD is mounted on the camera port of a wide field fluorescence microscope while the output image of the intensifier is optically coupled to a monochrome camera.

The II18MD is provided with control unit containing the high voltage power supply and RF amplifier. The control unit has a low voltage input to receive the external modulation signal to be amplified and biased with a variable DC cathode voltage. Adjusting the MCP voltage controls the intensifier gain. To prevent damaging the intensifier an overload protection circuit switches off the intensifier when the light output becomes too high.

To build a complete set-up for lifetime imaging, a compatible modulation signal generator, modulated light source and dedicated image processing software are available from Lambert Instruments.

Application:

• Protein interaction studies using GFP, CFP and YFP fusion constructs. Interaction is accompanied by direct energy transfer from donor to acceptor molecule (FRET) and is detected by a decrease in fluorescence lifetime of the donor molecule in the lifetime image.