High throughput analysis supercritical uid chromatography

3 Supercritical Fluid · SFC

The physical characteristics exhibited by supercritical uid include a di usion coe cient that is a hundred times greater than liquid and a viscosity that is at least one gure small- er. A supercritical uid chromatography system, such as a mobile phase medium, can rapidly perform separations without any drop in separation e ciency, even at fast ow rates, due to a rapid mass transfer inside the column when compared with high-speed liquid chromatography. In addition, when carbon dioxide is used as the medium, gasi- cation will occur keeping the separated and fractionated sample at a constant tempera-ture, making this technique capable of highly e cient rening with few post-processing hassles, such as the elimination of solvents after preparative isolation. This o ers a host of advantages, including cost cuts related to the expense of purchasing and discarding organic solvents, high throughput analysis, and rapid preparative isolation over a short period of time. As a gradient elution technique, SFC is capable of varying the three parameters of pres-sure, temperature, and modier solvent volume when separating oligomers and con- stituents with di ering characteristics.