TemPro - Stand-alone Benchtop, Filter-based Spectrofluorometer
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Catalog excerpts

TemPro - Stand-alone Benchtop, Filter-based Spectrofluorometer - 1

ELEMENTAL ANALYSIS FLUORESCENCE FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS PARTICLE CHARACTERIZATION RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING TemPro Fluorescence lifetime system Affordable fluorescence lifetime spectroscopy for every laboratory TemPro lifetime spectrofluorometers, unique in their sensitivity and selectivity, allow researchers to record fluorescence lifetimes, offering a dynamic picture of a sample’s characteristics, including molecular motion and size, solvation environment, and intermolecular distances—information that steady-state fluorescence cannot provide. They also offer the ability to temporally separate similar fluorescence spectra from two distinct species in a solution. TemPro uses Time-Correlated Single-Photon Counting (TCSPC)—the reliable, intuitive, and most sensitive lifetime-measurement technique. The filter-based TemPro can measure a complete range of lifetimes from as short as 100 picoseconds to 1 second, with our NanoLED and SpectraLED solid-state pulsed excitation sources spanning wavelengths from the UV to NIR. Applications Stern-Volmer quenching FRET (Förster Resonance Energy Transfer) Efficiency of photosynthesis Deconvolution of mixing processes Fluorescence anisotropy Fluorescence decay of 10–5 M aqueous Erythrosin B, taken with the TemPro, giving a lifetime of 85 ps. Excitation = 488 nm; emission > 550 nm. The green line is the mono-exponential fit to the decay data (red dots) after reconvolution with the instrumental response (blue dots). Anisotropy decay of 10 M DPH in Kaydol®, recorded with the TemPro. Excitation = 373 nm; emission > 418 nm. The initial anisotropy of 0.33 drops to zero showing a free rotation with 4.9 ns correlation time. –5 Protein denaturation

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TemPro - Stand-alone Benchtop, Filter-based Spectrofluorometer - 2

HORIBA Scientific specializes in optical instrumentation. The compact Tempro is designed for value and power, making it perfect for basic research and analytical measurements. Advanced Features Lifetime determination from 100 ps to 1 s* Detector response from 185-650 nm (others optional) Single-photon counting detection Data-acquisition and analysis software Fast, reliable USB 2.0 data link * Dependent on source, sample, and detector Span 100 ps to 1 s with the twist of a dial using TemPro's intuitive data-acquisition and lifetime-analysis software, NIR extended range detector (to 850 nm)...

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