HI84185
ISE AmmoniaNitrogen Meter
· Quick analysis· Small bench space
· Built-in electrode holder >
Significance of Use
The nitrogenous compounds of must and wineare deriving from grapes and play an important
role in fermentation, clarification, and potential
microbial instability of wines. They are pro-
foundly modified during the alcoholic fermen-
tation by the physiologic activity of yeast. Thus,
yeast assimilates 60-70% of the must nitrogen,ammonium ion completely disappearing dur-ing the fermentation and the total nitrogen
being slightly reduced.Ammonia is present in grapes as ammonium ina few milligrams amount and it serves as the
primary form of available nitrogen for yeast
metabolism. So, the content of ammonium ion
can drastically decrease during the alcoholic
fermentation, increasing again, especially in red
wines, at the end of the malolactic fermentationbecause the lactic bacteria releases ammonianitrogen in wine. The amount of ammonium
ion in must influences the rapidity of fermenta-
tion start and evolution.The ammonia concentration ranged from 24 to209 mg/L (ppm) in grapes and from a few mg/L
(ppm) to about 50 mg/L (ppm) in wine.The HI 84185 ISE Ammonia Nitrogen metermeasures the ammonia nitrogen (N-NH HI 84185 is supplied with reagents for 20 tests, HI 61101 Fluoride combination ISE electrode, membrane, HI 5315 reference half cell, HI 7665-T
temperature probe, 1000 µL automatic pipette, fillingsolution,
industrial tweezers, 2 plastic beakers (50 mL), magneticstir bar, 1 mL syringe, power cable and instruction manual. >
Electrodes, Solutions and
AccessoriesOrdering information
Electrodes HI 7662T Temperature probe HI 61101 Ammonia electrode Solutions Reagents HI 84185-20 Reagent set for 20 tests HI 84185-0 ISA solution for ammonia analysis in wine HI 84185-1 Standard solution No.1 for ammonia analysis in wine, 500 mL HI 84185-2 Standard solution No. 2 for ammonia analysis in wine, 500 mL Electrode Maintenance Cleaning and Storage Solutions )content in wine using an ion selective electrode. The used method is double standard
addition, a simple and rapid method of analysis,
and the result is readily displayed in ammonia
nitrogen (N-NH HI 4000-47 Buffer replacement kit >
3 HI 4000-40 Ammonia filling solution HI 4000-45 Ammonia conditioning and storage solution Accessories ) mg/L (ppm). HI 4001-51 (20) Membrane >
3 HI 740036P (10) 50 mL plastic beakers HI 731316 Stir bar (5) HI 731341 Automatic pipette 1000 µL HI 731351 (25) Tips for 1000 µL automatic pipette HI 740143 (6) Syringe 1mL HI 740144 (6)Syringe tip HI 741355P Capillary pipette >
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