NanoSIMS 50 in biological field. - CAMECA - #1

№CAMECA NanoSIMS 50

SCIENCE & METROLOGY SOLUTIONS

Jbcused beam

50nm sp a tial re so lutio n fo r sharp, iso to p e subc ellularortissue imaging

Parallel collec tion of up to 7 species (ex: ¹²C, ¹³C, ¹⁴N, ¹⁵N, ³²S, ³³S, ⁸¹Br) fo r simulta ne o us iso to p ic and/ortrace elemental analysis

Unambiguous iso tope markerloc alization and high se nsitivity fo r sub c e llula r iso to p ic ra tio measurements

Cultured endothelial cell image by c ourtesy of C. Lechene , MD, M.A. Schwartz, PhD, Harvard Medic al Sc ho ol, Brig ham & Women's Ho spital(IWMS, NCRR, NIH), Boston, MA., Sc rip ps Re search Institute, la Jo la, CA, USA.

The NanoSMS 50 is based on an original design by Pr. So dzian, University of Paris Sud.

Cs⁺ or O" primary ions are focused and rastered on the surface of the sample, at normal incidence. The ions sputtered by this primary beam are collected, and this secondary bn beam is shaped in order to be mass analyzed. The short working distance of the probe forming lens/extraction ensures good spatial resolution (<50nm) and high sec ondary bn c ollec tion effic iency. The double focusing Mattauch-Herzorg-like geometry and optimized transfer optics guarantee high transmission at high mass re so lutio n, re q uire d for ana lysis of small volumes ofbiological material. Up to seven ionic species can be simultaneously recorded, originating from the exact same sputtered volume, ensuring reliable isotopic ratio and perfect distribution compariso n.

ENA and DNA c o -loc alization by pulsing rat embryo fibroblasts with 15-N-uridine and bromo-deoxyuridine. Imaging of 14-N, 15-Nand 81-Br.

Pog ht: iso to p e coloroverLay (red+green = yellow). Eeld:20um x20um. Co urte sy C. lechene and & Eo, Ha rva rd Me die al School*.

'Images of rat embryo fibroblasts, hair section and mouse cochlea were obtained by Prof. Claude Lichene at Harvard MedicalSehool, Brig ham & Women'sHospitaL Boston, MA, USA.

lab oratory fund e d a s a National Re so urc e for Ima ging Mass Spectrometry by NIRR, NIH http 7/www .nrims.hms.harvard.edu/

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