Optosplit II - CAIRN RESEARCH - #9

Introduction

In fluorescence imaging applications it is often useful to acquire simultaneous images at two different emission wavelengths. Traditionally such applications have been restricted by the speed of an electronic filter changer, or by the cost and complexity of adding a second camera to a system. In many instances the region of interest does not require the full resolution of the camera so the ideal solution would be to simultaneously image at two different wavelengths on the same camera chip. In conjunction with a research microscope and a suitable CCD camera the Cairn Optosplit allows the researcher to do exactly this.

The Optosplit is usually supplied with a rectangular aperture to define an ROI and includes controls to allow the two images to be positioned accurately and conveniently within the camera frame. Images can be acquired using any imaging software and processed either manually off-line or using an appropriate analysis tool such as the Splitview module in Universal Imaging's Meta series software or Field Split in Andor Bioimaging's iQ.

As supplied, the instrument is configured to attach to the c-mount output port of a research microscope, with a c-mount CCD camera fitted to its output. The design allows for connection to a variety of alternative devices, so please consult with us if you intend using it in any other configuration.