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de novo sequencing is performed with the RapiDeNovo module. Subsequent MS-BLAST homology search is supported. • BioTools features batch result reporting in MTP viewer and HTML format, and „copy and paste“ export of displayed items, e.g. spectra, sequence maps, and search results, into reports and offi ce documents.

Brochure BioTools 2.2 - 30029 Key Features References

• Protein identifi cation is supported as a batch process, using peptide mass fi ngerprint (PMF), MALDI- and ESI-MS/MS data.• Effective viewing and judgment of individual PMF and MS/MS results is possible giving interactive access to candidate lists, spectra, informa- tion from database searches and the sequence viewer with coverage maps.• Sets of suspected modifi cations, tol-erated sequence errors and unspecifi c enzyme cleavages can be defi ned in order to interpret MS/MS data which could not be matched to a suspected protein sequence. Proposed structures are automatically scored by matching them to the experimental data. • Screening tools are provided for mutations and post-translational modifi cations, and modifi cation pro-fi les in recombinant proteins. • Known protein sequences can be compared to MS and MS/MS data, e.g. for QC of recombinant proteins.• BioTools is a core component of WARP directing the sample and data fl ow depending of the protein identi-fi cation results after each analytical step.• Interactive [1] W. Gish (1996-1999), http://blast.wustl.edu[2] A. Shevchenko, S. Sunyaev, A. Loboda, A. Shevchenko, P. Bork, W. Ens, K.G. Standing, Anal. Chem. 2001, 73 (9),1917-26
Fig. 11: RapiDeNovo allows consideration of modifi cations.