Revolution

Revolution
1 / 23 PagesView full catalog

Revolution

Product catalog summary
Introduction
The document provides an overview of the Andor Revolution WD and XD systems, which are advanced solutions for live cell imaging using confocal spinning disk technology. These systems are designed to capture high-resolution images with high sensitivity and speed, utilizing technologies such as sCMOS and EMCCD cameras.
System Overview
The Revolution WD and XD systems are built around the Yokogawa CSU spinning disk technology, known for its high speed and low phototoxicity. The WD system offers a large field of view and is optimized for thick samples, while the XD system is tailored for high-speed, high-sensitivity imaging at high magnifications.
Key Features and Benefits
  • Yokogawa CSU Spinning Disk: High speed and low phototoxicity.
  • iXon EMCCDs: High sensitivity for detecting weak signals.
  • sCMOS Cameras: High resolution and large fields of view.
  • Borealis Enhancement: Improves image quality and extends excitation range.
Applications
The systems are suitable for research fields such as developmental biology, stem cell research, neuroscience, and plant research, supporting imaging techniques like FRAPPA, TIRF, and optogenetics.
System Configurations
  • Revolution WDi: Versatile solution for inverted microscopes.
  • Revolution XDi: High-speed configuration for inverted microscopes.
  • Revolution XDh: Combines XD system with an upright microscope.
  • Revolution XDv: Compact solution for upright microscopes.
Customization and Flexibility
Andor offers bespoke solutions tailored to specific customer needs, integrating existing hardware for cost efficiency and providing custom mechanical and optical designs.
Conclusion
The Andor Revolution systems provide advanced, flexible solutions for live cell imaging, offering high performance and adaptability to meet diverse research needs.
Customer Special Request Process
Andor acknowledges that standard products may not meet all customer requirements. Custom configurations, like the Revolution XD, allow integration of live cell imaging with laser tweezers, enhancing research capabilities.
Microscopy System Cameras
EMCCD technology has advanced spinning disk confocal microscopy by reducing noise and enhancing sensitivity. The Neo sCMOS camera complements the iXon range with high speed and low read noise.
Software and Hardware Compatibility
Andor products are compatible with various software solutions, providing flexibility in data acquisition and analysis. The iQ software offers a user-friendly interface for multi-dimensional imaging.
Microscopy System Components
The CSU-X1 and CSU-W1 confocal spinning disk units offer high-speed imaging with low phototoxicity.
Advanced Illumination Systems
Andor's FRAPPA and MicroPoint systems offer advanced photo-bleaching and activation capabilities.
Precision Control and Automation
The Precision Controller Unit (PCU) provides system synchronization and control for external devices.
Light Sources and Dual Camera Solutions
A range of illumination sources, including LEDs and lasers, support high brightness and rapid switching.
Spinning Disk Confocal Microscopy Applications
  • Chromosome Spindle Attachments: Used to study kinetochore attachments in Drosophila cells.
  • Gene Territories in Yeast: Observes gene territories in live yeast cells.
Technical Insights
Spinning disk confocal systems offer superior spatial resolution, real-time high-contrast imaging, and reduced phototoxic effects.
Conclusion
The use of spinning disk technology provides significant insights into cellular processes, enhancing imaging capabilities and reducing phototoxicity.
Gene Territories and Nuclear Architecture
Research shows that gene positions within the nucleolus are critical for determining gene activity.
Spinning Disk Confocal Microscopy
Offers advantages over conventional laser scanning confocal microscopy by using multiple points of illumination simultaneously.
Borealis Technology
Enhances the Yokogawa Confocal Spinning Disk Unit by improving light throughput and uniformity.
Conclusion
Advancements in spinning disk confocal microscopy and Borealis technology significantly improve imaging performance.
Fluorescence Anisotropy in Microscopy
Discusses the use of fluorescence anisotropy in microscopy, particularly in live cell imaging.
Anisotropy and Homo-FRET
Anisotropy refers to the unequal distribution of fluorescence in different directions, used to study molecular interactions.
Polarization and the CSU
Identifies issues with laser polarization degradation in CSU products and presents a solution involving a high-performance polarizer.
Confocal Dual Camera Anisotropy Imaging
A dual camera system allows simultaneous detection of orthogonal polarizations, improving resolution and dynamic range.
Conclusion
The SpiDA system enhances real-time confocal anisotropy imaging.
Scientific Imaging Technology
Compares sCMOS, Interline CCD, and EMCCD technologies, highlighting the unique capabilities of sCMOS technology.
Current Scientific Imagers
Interline CCDs offer high performance at a low cost, while EMCCDs provide high sensitivity through on-chip amplification.
sCMOS Technology
Offers low noise, high speed, wide dynamic range, and high resolution without the trade-offs seen in other technologies.
Comparative Analysis
sCMOS shows superior performance in noise, speed, dynamic range, and resolution compared to Interline CCD and EMCCD technologies.
Conclusion
sCMOS technology represents a leap forward in scientific imaging, offering a balanced combination of performance parameters.
See more

Catalog excerpts

Revolution-1

an Oxford Instruments company Confocal Spinning Disk Solutions

 Open the catalog to page 1
Revolution-2

Meet the family Experience tells us that almost every customer is unique in their requirements, and so rarely are two Revolution systems the same. We pride ourselves in offering flexibility so that you can focus on your applications. Revolution XDi The classic high speed configuration for inverted microscopes Revolution XDh Combine XD system choice with an upright microscope Revolution XDv A focused upright solution when lab space is at a premium The only solution when life is so precious The Andor Revolution WD and XD form a family of flexible system solutions focused on live cell imaging. At...

 Open the catalog to page 2
Revolution-3

The Revolution WD is the latest spinning disk confocal system in Andor’s range. It is the ultimate solution for the diverse range of samples used in live cell imaging. Using the CSU-W1 from Yokogawa, the Revolution WD retains the pedigree expected from the Revolution systems, but offers so much more in order to meet your research needs. TuCam CSU OptoSplit KEY APPLICATIONS Regenerating cholinergic motorneurons in the nematode C.elegans. Upper Image - Dual labelled nematode. Collaboration with T. Edwards, Hammarlund Laboratory, Yale School of Medicine. Camera Piezo Stage Developmental biology...

 Open the catalog to page 3
Revolution-4

The Revolution XDi is based around an inverted microscope, the most common choice for live cell imaging. With a disk speed that supports up to 2,000 frames per second, it is the primary choice for high speed cellular dynamics and applications such as ion imaging. It delivers premium performance for speed and sensitivity at high magnifications. Power and flexibility with Borealis NEW Borealis offers many benefits resulting from its highly uniform field of illumination and improved light throughput. These include enhancements in: image quality, quantitative analysis and axial fidelity. Borealis...

 Open the catalog to page 4
Revolution-5

Full Featured Upright Microscope System XDh has been designed to allow those who work on an upright microscope to benefit from the capabilities available from our full component range. The XDh is suitable for applications such as plant biology research, and in developmental biology models such as c.elegans and drosophila. In these cases, whilst an inverted microscope configuration is possible, an upright microscope can be more practical owing to the sample preparation with a glass coverslip more typically on the upper facing surface. Other examples of applications requiring an upright microscope...

 Open the catalog to page 5
Revolution-6

Upright Scope with Reduced System Footprint Filter Wheel The Revolution XDv offers you a compact solution on an upright microscope. In keeping this system footprint small, it is well suited to labs with limited space or to fit inside a Faraday cage for electrophysiology rigs. In securing the CSU-X, filter wheel and camera to our vertical mount, you are guaranteed complete stability. Laser 4 Laser 5 Laser 6 KEY APPLICATION EXAMPLE - ELECTROPHYSIOLOGY With the Revolution XDv you can confidently combine spinning disk confocal microscopy with techniques as sensitive as patch clamping a cell. Spinning...

 Open the catalog to page 6
Revolution-7

Revolution Custom Systems The flexibility for me to define a novel configuration for the Revolution XD allowed me to combine live cell imaging with laser tweezers. This offers new avenues of investigation for my research. At Andor we realize that, sometimes, even our adaptable and flexible off-the-shelf products are not enough to meet some of the more demanding application requirements of our customers. For this reason we provide a bespoke service to our customers, whereby a dedicated highly experienced team of engineers and application specialists provide customer specific solutions. The process...

 Open the catalog to page 7
Revolution-8

Microscopy System Cameras EMCCD technology revolutionized spinning disk confocal microscopy. The ability to cool to -100°C and the use of EM gain effectively eliminates noise and provides routine single photon sensitivity. The key benefits of Andor’s iXon EMCCD cameras are: The Neo sCMOS camera is the perfect partner to the iXon range. Though not as sensitive as EMCCD cameras, sCMOS runs at high speeds and has extremely low read noise at 1 e-/pix/sec, giving excellent signal to noise performance. The key benefits of Neo sCMOS are: • Extreme sensitivity for working at low levels of sample illumination,...

 Open the catalog to page 8
Revolution-9

Software MicroManager Flexibility in your choice of software as well as hardware. Instrument Control and Data Acquisition NEW Andor iQ Andor’s iQ software is designed for multi-dimensional and multi-modal imaging. With years of experience and development behind it, iQ offers immense flexibility for a wide range of live cell imaging requirements. iQ3, the very latest version, introduces the 6D GUI (Graphical User Interface) and a user management system. protocols from other users, even from previous iQ versions. This will be of great benefit to core facility managers, helping them to manage users...

 Open the catalog to page 9
Revolution-10

Microscopy System Components Confocal Spinning Disk Unit Confocal Spinning Disk Unit The CSU-W1 offers complete versatility, addressing a wide range of sample types due to an alternative disk design to the CSU-X1. Choose up to 2 disk patterns according to your requirements. Available in 3 models: single and dual camera, and split-view, the CSU-W1 offers a range of options according to your budget or application requirements (e.g. simultaneous dual wavelength capture). Upgrade single camera versions in the future with TuCam if you wish. (see page 23) The CSU-X1 from Yokogawa is the premier solution...

 Open the catalog to page 10
Revolution-11

Microscopy System Components Rotr™ Filter Wheel - Emission discrimination Mosaic 3 - Active Illumination Our systems use filter wheels to select by wavelength or polarisation. Our new highspeed Rotr filter wheel offers wavelength switching up to nine frame pairs/sec when fully loaded with filters (manufacturers quote unloaded times). Multiple filter wheels are sequential. The Mosaic active illumination system utilizes digital mirror device (DMD) technology to control the illumination field of a fluorescence microscope. Using laser or arc lamp sources, Mosaic achieves real time and near diffraction...

 Open the catalog to page 11
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.