Multi-Wavelength Imaging
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Multi-Wavelength Imaging Solutions For Simultaneous Capture

Multi-Wavelength Imaging Solutions For Simultaneous Capture The vast majority of fluorescence microscopy applications involve the use of more than one fluorescent probe e.g. FRET, dual color imaging, co-localization studies etc. For simultaneous multi-wavelength imaging Andor offers the most flexible and versatile solutions available, either splitting the wavelengths across two separate cameras or across adjacent sensor halves of a single camera. Image courtesy of Dr. Ulrike Engel, Nikon Imaging Centre, University of Heidelberg, Germany The TuCam, a new generation two-camera adaptor, for...

TuCam Dual Image Adapter Andor’s TuCam is a new generation twocamera adapter for macro or microscopic imaging applications. Available in C-mount, TuCam’s features include large aperture, exceptional transmission, very low distortion and high precision alignment using kinematic cassettes. The TuCam can be configured for simultaneous imaging from two similar cameras or as a switch between camera models with different imaging capabilities, including CCD, EMCCD, ICCD and sCMOS from Andor, and other third party detectors. Since separate wavelengths can be split across two individual cameras, the...

Dual Image Adapter Transmission Curve for C-mount TuCam Andor’s TuCam utilizes lenses with broadband anti-reflection coatings specifically chosen to maximize system throughput in the 400 - 750 nm wavelength band. This is a typical performance for these instruments and may vary slightly between individual units. Beam splitter optics are not included. This schematic of the optical path shows the emission beam (yellow) coming from the microscope and entering the TuCam via the C-mount attachment point. When this emission beam enters the filter cassette, which contains a dichroic mirror, the...

Optosplit II One Camera Solution - Dual Emission Image Splitter The Optosplit II image splitter is an elegant device that divides the image into two separate, spatially equivalent components that can be displayed side by side on a camera sensor, enabling a single camera to record images simultaneously at two different optical wavelengths. The Optosplit has been designed as a convenient, inexpensive solution to simultaneous imaging. Splitting is usually performed on a basis of wavelength, allowing applications such as ratiometric ion imaging or FRET, however, polarizing beamsplitters are...

Optosplit III One Camera Solution - Triple Emission Image Splitter The Optosplit III, a three-way image splitter, is a simple device for dividing an image into two or three separate, spatially equivalent components. These can be displayed side by side on a camera sensor, enabling a single camera to record images simultaneously at three different optical wavelengths. pause acquisition while the filter position is changed. This allows your camera to be operated at the fastest capture rates it is capable of achieving. Polarization Fluorescence Resonance Energy Transfer Single camera A cost...

Application and Technical Notes For multi-wavelength imaging, Andor has the most flexible and versatile solutions available to the researcher. The following section is dedicated to providing a greater depth of understanding to the functionality of the multi-wavelength imaging products available from Andor. The content in the subsequent pages will illustrate how the TuCam can be used with different detectors in a range of software, making it easy to integrate into any laboratory. In addition, you will read a summarized publication where the Optosplit II, in combination with the iXon3 EMCCD...

Application Note Multi-color direct STORM with red emitting carbocyanines Lampe et al have developed a novel variant of direct STORM (dSTORM) termed spectral demixing dSTORM (SD-dSTORM) that combines the photochemical advantages of the red emitting carbocyanine dyes with the principal of spectral demixing. Specifically, they use a novel combination of carbocyanine dyes for super resolution, Alexa Fluor 647 and Alexa Fluor 700, which both show the excellent buffer compatible blinking characteristics needed for single molecule localization. SD-dSTORM requires reduced laser power and fewer...

Technical Note Dual Wavelength Imaging using TuCam: Software Set-up Focus This technical note is provided to aid the user in the set-up of their software for use with TuCam. The example softwares covered are Andor Solis, Andor iQ and MetaMorph from Molecular Devices Corporation. There are a number of key up-front considerations: 1. Does the software support the detectors under consideration? 2. Can the software be set up to acquire images simultaneously from more than one camera? 3. Is the software capable of operating two cameras within one instance of the software, or is it necessary to...

Part Numbers Technical Note Software recommendations for acquisition and analysis of dual wavelength microscopy images Base Unit Configurations TuCam S-CMT The following table summarizes the software packages that are compatible with Andor’s cameras when operated under simultaneous dual camera acquisition. 2 x Neo 5.5 sCMOS Standard Filter Sets Any C-mount device including microscope or lens TR-EMFS-F01 When acquiring two color images with two cameras on TuCam it is essential that the acquisition software is compatible with the detectors used and is capable of acquiring simultaneous image...

Customer Support Andor products are regularly used in critical applications and we can provide a variety of customer support services to maximize the return on your investment and ensure that your product continues to operate at its optimum performance. Andor has customer support teams located across North America, Asia and Europe, allowing us to provide local technical assistance and advice. Requests for support can be made at any time by contacting our technical support team at andor.com/support. Andor offers a variety of support under the following format: • On-site product specialists...