TN001 Bravo 96-Channel 10uL Tip Accuracy and Precision - Agilent Technologies - Life Sciences and Chemical - #1

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Technical Note Velocity11 Bravo™ Liquid Handling Platform: 96 Channel l0 u L Tip Accuracy and Precision Introduction Automated liquid handling devices are used in many processes performed in laboratories to­day. Liquid handling automation increases sam-ple processing and throughput(l) and improves accuracy (desired dispensed volume is equal to the actual dispensed volume) and precision (a narrow distribution of dispensed volume) when compared to a person operating a handheld liquid pipettor. Automated liquid handling de­vices can also significantly reduce the occur­rence of errors in a process(2). To further the effort to automate routine laboratory processes, Velocityll® has developed the Bravo liquid han-dling platform. Bravo Features The Bravo has nine deck positions which accom-modate any 96-, 384-, or l536-well SBS standard microplates. Deck positions can be configured with heating, cooling, and shaking stations, as well as maintaining open locations for tip boxes, sample microplates and reservoirs. The Bravo has nine deck positions and can be configured with interchangeable 8-, l6-, 96-, or 384- fixed and disposable tip heads. The Bravo can be used with 8-, l6-, 96-, or 384-channel fixed tip or disposable tip heads. Heads can be interchanged in a matter of seconds. The Bravo is designed to be used either standalone or as part of a robotic laboratory automation system. It is also designed to fit inside some models of laminar flow hoods and retain laminar flow, thereby opening up cell-based plating and cellular assays to an automated liquid handling platform. Accuracy and Precision Testing This technical note describes a method to meas-ure the accuracy and precision of the Bravo in conjunction with a 96 Channel ST Disposable Tip Head. The l0 u L disposable tips were tested at the lower end of the practical volume range (0.3­2 uL). Performance at volumes >2 uL meets or exceeds the 2 uL performance. Measurements are determined by dispensing a tartrazine/DMSO solution into dry microplates, filling the plates with water, and measuring tartrazine absorbance. The product's performance meets or exceeds CVs of 5% and accuracy of ±l0% of the desired volume across the practical volume range. Summa Bravo, 96ST head, l0 u L tips Materials Velocityll Bravo with a 96 Channel ST Disposable Tip Head Velocityll 384ST l0 uL tips (product no. l0734.l02) Velocityll 96-well manual fill reservoir (product no. 08l05.00l) 384-well polystyrene, flat bottom plates (Greiner 78ll0l ) Tartrazine solution, 0.25% (w/v) dissolved in dimethylsulfoxide [DMSO] UV/Vis Spectrophotometer with a 405 nm filter (Thermo® Multiskan Ascent )